Serial pyrosequencing for quantitative DNA methylation analysis

Serial pyrosequencing for quantitative DNA methylation analysis.

BioTechniques 721 In mammals, DNA methylation is a genetically programmed DNA modification and occurs almost exclusively at the 5-position of the pyrimidine ring of cytosines. Cytosine methylation is essential for mammalian embryogenesis and development, implicated in X inactivation, and influences gene expression of the corresponding genes, giving rise to distinct expressions patterns in diffe...

Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites.

DNA Methylation of GSTP1 in Human Prostate Tissues: Pyrosequencing Analysis

PURPOSE DNA methylation is an important epigenetic mechanism of gene regulation and plays essential roles in tumor initiation and progression. Differences in methylation patterns between neoplastic and normal cells can be used to detect the presence of cancer. The aim of the present study was to evaluate the usefulness of glutathione-S-transferase-Pi (GSTP1) hypermethylation in discriminating b...

A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results

Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin's expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin's receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing...

dna-directed methylation analysis methodology

epigenetics alterations, especially dna methylation, play a critical role in control of gene expressions. abnormal patterns of methylation are observed in early-stages of many cancers. therefore, methylation analysis is useful in primary detection of tumors. advances knowledge about the functional role of aberrant epigenetic modifications as potential biomarkers for cancer, have attracted consi...